VCE Masterclass: Biology UNIT 3: Photosynthesis and Biochemical Pathways

Booked OUT for 2026

Step into the role of a scientist

The KIOSC VCE Biology Investigation Program is designed to support schools in delivering Unit 3 Biology – Area of Study 3 (AOS3). This hands‑on program provides students with the time, space, and specialised equipment needed to design and conduct their own scientific investigation linked to photosynthesis.

Using living algal systems, students explore how environmental factors — such as light intensity or temperature — influence the rate of photosynthesis. This program aligns directly with the skills and outcomes required in the VCE Biology Study Design and helps students build confidence in experimental design, data collection, analysis, and scientific communication.

 

Purpose of the Program

This program helps students:

  • Develop a deeper understanding of cellular processes through practical investigation
  • Apply the scientific method in a controlled laboratory environment
  • Connect theory from Unit 3 and Unit 4 with real‑world inquiry
  • Prepare the foundation for their Unit 3 scientific poster assessment

The introductory video series on the website outlines the program’s purpose, expectations, and the scientific inquiry process students will follow.

 

Before Your Visit

Step 1: Watch the Introduction Video (Click on this link)

Step 2: Watch the Planning and Methodology Video (Click on this link)

Step 3: Watch and complete the Pre-Work ONLINE activity (Click on this link)

This online task has pre-work activities on photosynthesis, relating to the experiment you will be designing and conducting. It also has a section on how to use the KIOSC equipment. This is vital to ensure a smooth transition in conducting your task.

 

Step 4: Students will need to arrive with a teacher‑approved experimental plan, including:

✅ Variables

Independent, dependent, and controlled variables

✅ Aim

A statement describing what their investigation seeks to determine

✅ Hypothesis

A clear prediction linking the independent and dependent variable

✅ Materials

Based on the equipment demonstrated in the preparation videos

✅ Method

A draft procedure outlining the steps they intend to follow
(Students may refine this after practice activities at KIOSC)

✅ Results Plan

How students will:

  • Collect data (qualitative and/or quantitative)
  • Record it (tables, observations)
  • Represent it (graphs or visual displays)

This preparation ensures students use their laboratory time productively and meaningfully.


The Investigation System: Algal Balls

Students conduct their investigation using algal balls — algae encapsulated in alginate, providing a safe, stable, and consistent system for measuring photosynthesis.

Hydrogen Carbonate Indicator solution allows students to track CO₂ levels through colour change:

  • Purple → Increased pH, indicating photosynthesis
  • Yellow/orange → Decreased pH, indicating respiration

This highly visual method helps students connect cellular processes to observable changes, reinforcing conceptual understanding.


What Happens During the 3‑Hour Session

1. Equipment Training

KIOSC educators guide students through:

  • pH probes
  • UV spectrophotometers
  • Light and temperature control equipment
  • Colour indicator charts

This ensures all students can confidently handle laboratory tools.

2. Familiarisation Activity

A short guided experiment allows students to practise:

  • Handling algal balls
  • Observing indicator colour changes
  • Identifying sources of uncertainty
  • Refining their method before beginning their independent investigation

3. Independent Investigation Time

Students spend 1–2 hours carrying out their self‑designed experiments.
They are encouraged to repeat trials where possible to improve reliability.

4. Debrief and Poster Preparation Guidance

If time permits, the KIOSC team supports students in:

  • Interpreting results
  • Considering sources of error
  • Understanding how their findings connect to the scientific poster they will complete at school

How This Program Supports Schools

This experience:

  • Meets VCE Biology requirements for a student‑designed scientific investigation
  • Provides access to specialised equipment schools may not have
  • Reinforces scientific inquiry skills needed for Units 3 and 4
  • Builds student independence, confidence, and scientific reasoning

KIOSC supplies the laboratory environment, expert guidance, and hands‑on equipment training.
Teachers retain full oversight of student progress, assessment, and approval of investigation plans.

Curriculum Focus

VCE Biology Unit 3 – AOS 1: Outcome 2: Photosynthesis as an example of biochemical pathways

Regulation of biochemical pathways in photosynthesis and cellular respiration
1.1 The general structure of the biochemical pathways in photosynthesis and cellular respiration from initial reactant to final product
1.2 The general role of enzymes and coenzymes in facilitating steps in photosynthesis and cellular respiration
1.3 The general factors that impact on enzyme function in relation to photosynthesis and cellular respiration: changes in temperature, pH, concentration, competitive and non-competitive enzyme inhibitors
Photosynthesis as an example of biochemical pathways
2.1 Inputs, outputs and locations of the light dependent and light independent stages of photosynthesis in C3 plants (details of biochemical pathway mechanisms are not required)
2.2 The role of Rubisco in photosynthesis, including adaptations of C3, C4 and CAM plants to maximise the efficiency of photosynthesis
2.3 The factors that affect the rate of photosynthesis: light availability, water availability, temperature and carbon dioxide concentration